dda/tdb liposomes containing soluble leishmania major antigens induced a mixed th1/th2 immune response in balb/c mice

نویسندگان

mansure hojatizade pathobiology department, school of public health, tehran university of medical sciences, tehran, iran

department of basic medical sciences, neyshabur university of medical sciences, neyshabur, iran

ali badiee nanotechnology research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran

ali khamesipour center for research and training in skin diseases and leprosy, tehran university of medical sciences, tehran, iran

چکیده

objective(s): leishmaniasis is a complex parasitic disease that represents a major public health problem. despite numerous attempts over the past decades, yet there is no effective vaccine against human leishmaniasis probably due to the lack of suitable adjuvants. in this study, a first generation liposomal-based leishmania vaccine was developed using soluble leishmania major antigens (sla) and á, ü-trehalose6, 6'-dibehenat (tdb) as an immunostimulatory adjuvant. in this liposome structure, the cationic lipid dimethyldioctadecylammonium (dda) provides intrinsic adjuvant activity and cholesterol was added as a membrane stabilizer. liposomes containing sla were prepared.materials and methods: balb/c mice were subcutaneously (sc) immunized with lip (dda/tdb/chol)-sla+, lip (dda/tdb)-sla+, lip (dda)-sla+, lip (dda/chol)-sla+, sla or tris-hcl buffer. immunization was done every two weeks for three weeks. the immunized mice were then challenged sc in the left footpad with 1×106 stationary phase l. major promastigotes (50 ìl), at 2 weeks after last booster injection.results: mice immunized with any of the liposomal formulations containing sla (lip-sla+), substantially increased footpad swelling and parasite loads of foot and spleen with no significant difference compared to tris-hcl buffer or sla alone. lip-sla+ formulations induced a mixed th1/th2 immune response characterized by ifn-ã and il-4 production as well as high levels of igg1 anti-leishmania antibody. conclusion: immunization with liposomes containing dda and/or tdb in combination with sla induces a mixed th1/th2 immune response and is not an appropriate strategy for preferential induction of a th1 response and protection against leishmaniasis.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Sphingomyelin Liposomes Containing Soluble Leishmania major antigens Induced Strong Th2 Immune Response in BALB/c Mice

  Objective(s): Soluble Leishmania antigens (SLA) provide suitable protection against leishmaniasis in murine model when delivered by an appropriate delivery system. Liposomes have been shown to be suitable vaccine delivery systems against leishmaniasis, however, the phospholipase-A (PLA) activity of SLA is a drawback to prepare a stable liposomal SLA. One strategy to overcome this problem migh...

متن کامل

DDA/TDB liposomes containing soluble Leishmania major antigens induced a mixed Th1/Th2 immune response in BALB/c mice

Objective(s): Leishmaniasis is a complex parasitic disease that represents a major public health problem. Despite numerous attempts over the past decades, yet there is no effective vaccine against human leishmaniasis probably due to the lack of suitable adjuvants. In this study, a first generation liposomal-based Leishmania vaccine was developed using soluble Leishmania major antigens (SLA) and...

متن کامل

sphingomyelin liposomes containing soluble leishmania major antigens induced strong th2 immune response in balb/c mice

objective(s): soluble leishmania antigens (sla) provide suitable protection against leishmaniasis in murine model when delivered by an appropriate delivery system. liposomes have been shown to be suitable vaccine delivery systems against leishmaniasis, however, the phospholipase-a (pla) activity of sla is a drawback to prepare a stable liposomal sla. one strategy to overcome this problem might ...

متن کامل

Sphingomyelin Liposomes Containing Soluble Leishmania major antigens Induced Strong Th2 Immune Response in BALB/c Mice

OBJECTIVE(S) Soluble Leishmania antigens (SLA) provide suitable protection against leishmaniasis in murine model when delivered by an appropriate delivery system. Liposomes have been shown to be suitable vaccine delivery systems against leishmaniasis, however, the phospholipase-A (PLA) activity of SLA is a drawback to prepare a stable liposomal SLA. One strategy to overcome this problem might b...

متن کامل

Cationic Immune Stimulating Complexes Containing Soluble Leishmania Antigens: Preparation, Characterization and in Vivo Immune Response Evaluation

Background: Cationic immune stimulating complexes (PLUSCOMs) are particulate antigen delivery systems. PLUSCOMs consist of cationic immunostimulatory complexes (ISCOMs) derivatives and are able to elicit in vivo T cell responses against an antigen. Objective: To evaluate the effects of PLUSCOMs containing Leishmaniamajor antigens (SLA) on the type of immune response generated in the murine mod...

متن کامل

cationic immune stimulating complexes containing soluble leishmania antigens: preparation, characterization and in vivo immune response evaluation

background: cationic immune stimulating complexes (pluscoms) are particulate antigen delivery systems. pluscoms consist of cationic immunostimulatory complexes (iscoms) derivatives and are able to elicit in vivo t cell responses against an antigen. objective: to evaluate the effects of pluscoms containing leishmaniamajor antigens (sla) on the type of immune response generated in the murine mode...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
nanomedicine journal

جلد ۴، شماره ۲، صفحات ۷۱-۸۲

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023